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Alternate day fasting ameliorates renal damage in rats received high fat and fructose diet through enhancement of renal autophagy, decreasing renal fibrosis and apoptosis

Article

Last updated: 24 Dec 2024

Subjects

-

Tags

Renal Physiology

Abstract

Background: A high-fat, high-fructose diet (HFFD) is known to increase one's risk for developing obesity, the metabolic syndrome, all of which contribute to the development of chronic kidney disease.Aim of the work: The goal of the current study is to clarify the biochemical and histological impacts of alternate-day fasting on renal disease brought on by a high-fat and fructose diet. Methods: 18 adult albino male rats weighing 200–250 grams were randomly assigned to three groups of six: Group 1 had a conventional diet, Group 2 received a high fat and fructose diet, and Group 3 received a high fat and fructose diet as well as alternate days of fasting. Fasting for 24 hours was applied in HFFD rats alternatively from the 9th week to the 12th week respectively. All rat groups were subjected to a histopathological study of the kidney to assess collagen deposition, LC3, α-SMA, and caspase 3 immuno-expression besides biochemical investigations to identify metabolic syndrome parameters as lipid metabolism, inflammatory markers, and oxidant-antioxidant status in the kidney tissues of all groups.Results: HFFD rats subjected to alternate-day fasting showed an improvement in adiposity, lipid metabolism, and cellular oxidative and inflammatory markers. Also, histopathological examination confirmed the valuable effect of fasting as evidenced by enhancement of renal autophagy, decreasing fibrosis and apoptosis.Conclusion: Our study's findings reveal that ADF from the 9th to 12th week improved metabolic disturbances and renal injury induced by HFFD. Fasting also significantly reduced inflammation and oxidative stress on both biochemical and histopathological levels.

DOI

10.21608/besps.2024.299683.1169

Keywords

alternate day fasting, high-fat diet/fructose, autophagy, Oxidative Stress, Immunohistochemistry

Authors

First Name

Khaled

Last Name

Elbayumi

MiddleName

S.

Affiliation

Department of Human Anatomy & Embryology, Faculty of Medicine, Mansoura University, Mansoura, Egypt

Email

khalad200772@mans.edu.eg

City

-

Orcid

-

First Name

Zienab

Last Name

Eldken

MiddleName

Helmy

Affiliation

Department of Physiology, Faculty of Medicine, University of Mansoura

Email

zienabeldken@yahoo.com

City

-

Orcid

-

First Name

Haitham

Last Name

Sewilam

MiddleName

M.

Affiliation

Department of Histology, Faculty of Medicine, Helwan University, Helwan, Egypt

Email

haitham.sewilam@gmail.com

City

-

Orcid

-

First Name

Rania

Last Name

Khalil

MiddleName

M.

Affiliation

Department of Biochemistry, Faculty of Pharmacy, Delta University for Science and Technology, Gamasa, Egypt

Email

rania742002@yahoo.com

City

-

Orcid

-

First Name

Ahmed

Last Name

Hamad

MiddleName

G.

Affiliation

Department of Human Anatomy & Embryology, Faculty of Medicine, Mansoura University, Mansoura, Egypt

Email

a_hammad@mans.edu.eg

City

-

Orcid

-

Volume

44

Article Issue

4

Related Issue

44934

Issue Date

2024-10-01

Receive Date

2024-06-26

Publish Date

2024-10-01

Page Start

196

Page End

211

Print ISSN

1110-0842

Online ISSN

2356-9514

Link

https://besps.journals.ekb.eg/article_382238.html

Detail API

https://besps.journals.ekb.eg/service?article_code=382238

Order

382,238

Type

Original Article

Type Code

567

Publication Type

Journal

Publication Title

Bulletin of Egyptian Society for Physiological Sciences

Publication Link

https://besps.journals.ekb.eg/

MainTitle

Alternate day fasting ameliorates renal damage in rats received high fat and fructose diet through enhancement of renal autophagy, decreasing renal fibrosis and apoptosis

Details

Type

Article

Created At

24 Dec 2024