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Ameliorative effect of resveratrol against type 2 diabetes in rats through triggering of autophagy and inhibition of mTOR pathway.

Article

Last updated: 03 Jan 2025

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Subjects

-

Tags

Endocrine and Reproductive Physiology

Abstract

Background: Type 2 diabetes mellitus (T2DM), which affects millions of individuals, has become a serious health problem. The mammalian target of rapamycin (mTOR) hyperactivation is a negatively involved autophagy that may in fact aid in the loss of β-cell function. Resveratrol (RSV) is a naturally occurring phytoalexin, mostly found in cereals, fruits, and vegetables that have antioxidant effects.
Aim: In the current work, Wistar rats exposed to high-fat diet (HFD) and streptozotocin (STZ)-induced T2DM were evaluated for resveratrol's protective effects on pancreatic functions, and mTOR-mediated autophagy.
Materials and Methods: Four equal groups of forty male Wistar rats were created at random. Group 1 (G1) was maintained in typical control circumstances and given a balanced diet; G2, G3, and G4 were fed HFD for 10 weeks continuously; at the end of the 6th week injected I/P STZ as a single dose to induce T2DM. Three days after diabetic induction, G3 was received RSV daily for continuously 4 weeks. G4 was received RSV in addition to Chloroquine (CQ) as autophagy inhibitor.
Results: The findings shown that RSV reduces the pancreatic dysfunctions brought on by HFD-STZ by inhibiting the mTOR pathway through decreasing the level of mTOR, Cas-3, and p62 and increasing the level of LC3. RSV activate autophagy and inhibit apoptotic-mediated cell death confirmed by transmission electron micrograph findings.
Conclusion: RSV is thought to protect pancreatic cells by inhibiting the mTOR pathway, which regulates the transition between autophagy and apoptotic machinery in diabetic circumstances.

DOI

10.21608/besps.2024.279336.1166

Keywords

autophagy, mTOR pathway, Oxidative Stress, Resveratrol, Type 2 diabetes mellitus

Authors

First Name

Lina

Last Name

Mohammed

MiddleName

Abdelhady

Affiliation

Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Benha University, Benha 13518, Egypt.

Email

lina.mohamed@fmed.bu.edu.eg

City

Benha

Orcid

0000-0002-6332-2977

First Name

Ahmed

Last Name

Hegazy

MiddleName

Medhat

Affiliation

Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh 13736, Qalyubia, Egypt.

Email

ahmed_medhat012@yahoo.com

City

-

Orcid

0000-0001-8606-5937

First Name

Walaa

Last Name

El Gazzar

MiddleName

Bayoumie

Affiliation

Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Benha University, Benha 13518, Egypt.

Email

bioch_2004@yahoo.com

City

Benha

Orcid

0000-0001-5172-1105

First Name

Noha

Last Name

El-Shaer

MiddleName

Osama

Affiliation

Department of Physiology, Faculty of Medicine, Benha University, Benha 13518, Egypt.

Email

noha.saad@fmed.bu.edu.eg

City

Benha

Orcid

0000-0001-6323-454X

First Name

Heba

Last Name

Bayoumi

MiddleName

-

Affiliation

Department of Histology and Cell Biology, Faculty of Medicine, Benha University, Benha 13518, Egypt.

Email

heba.bayoumi@fmed.bu.edu.eg

City

Benha

Orcid

0000-0002-0931-452X

First Name

Salwa

Last Name

Elgendy

MiddleName

A.

Affiliation

Deparment of Pharmacology, Faculty of Medicine, Benha University, Benha 13518, Egypt.

Email

salwaeg@yahoo.com

City

Benha

Orcid

0000-0003-4287-3099

First Name

Heba

Last Name

Elnoury

MiddleName

A.

Affiliation

Deparment of Pharmacology, Faculty of Medicine, Benha University, Benha 13518, Egypt.

Email

hebaalnoury81@gmail.com

City

Benha

Orcid

0000-0003-0513-8589

First Name

Hend

Last Name

Nasr

MiddleName

Elsayed

Affiliation

Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Benha University, Benha 13518, Egypt.

Email

hend.mosalm@fmed.bu.edu.eg

City

Benha

Orcid

0000-0002-7338-7627

Volume

44

Article Issue

3

Related Issue

44933

Issue Date

2024-07-01

Receive Date

2024-03-26

Publish Date

2024-07-01

Page Start

150

Page End

168

Print ISSN

1110-0842

Online ISSN

2356-9514

Article File

BESPS_Volume 44_Issue 3_Pages 150-168.pdf

PDF . 359.1kB

Link

https://besps.journals.ekb.eg/article_362462.html

Detail API

https://besps.journals.ekb.eg/service?article_code=362462

Order

362,462

Type

Review Article

Type Code

574

Publication Type

Journal

Publication Title

Bulletin of Egyptian Society for Physiological Sciences

Publication Link

https://besps.journals.ekb.eg/

MainTitle

Ameliorative effect of resveratrol against type 2 diabetes in rats through triggering of autophagy and inhibition of mTOR pathway.

Details

Type

Article

Created At

24 Dec 2024