Montanide ISA50 and Aluminum hydroxide gel were used as adjuvant for preparation of FMD vaccine. The vaccines were tested in Guinea pigs and cattle.Serum neutralization test and indirect ELISA were carried out to follow up the immune status of vaccinated cattle for 30 weeks post vac­cination (WPV).The revealed results indicate that the vaccine emulsified with Montanide ISA50 ad­juvant appeared to be more potent and gave a higher level of immunity to the vaccinated cattle than that produced from AL (OH) 3 gel vaccine till the end of the 30 (WPV). Also that indicates the ability of Montanide ISA50 oil adjuvant vac­cine for serological responses toFMD antigens more than AL (OH) 3 gel adjuvant vaccine