A total of 196 milk samples were examined bac­teriologically for detection of methicillin resistant Staphylococcus aureus during the period from January to October 2004 and results obtained showed high incidence of isolation of S. aureus (38.46%) from a total 13 strains of staphylococci (29.54%). All obtained isolates were identified using a commercial kit system (API STAPH sys­tem consists of: Carrying out 19 tests using the API STAPH strip). Antibiotic sensitivity test were done using five antibiotics, which are frequently used in mastitis therapy were chosen: penicillin-G, ampicillin, ka­namycin, and cephalexine. The fifth compound was methicillin and results obtained showed no such strains could be found. S. aureus strains were susceptible to the antibiotics tested corn- prise 60 % (penicillin G), 80 % (kanamycin, cephalexine) and ampicillin (20 %). Pathogenicity of the isolates were discussed in­cluding both haemolysin titre and pathogenicity in mice. The results obtained revealed that major­ity of the S. aureus (80%) isolates were haemo­lytic with the maximum haemolytic activity (1/ 1024) and concerning pathogenicity in mice mor­tality rate was 100%. Genetic characterization of staphylococci by Pulsed Field Gel Electrophoresis (PFGE) analysis revealed no S. aureus resistance methicillin was recorded.