A total 600 milk samples were collected from the udder halves of 300 native dairy sheep and nannies at Haliab, Shalateen and Abu-Ramad. California mastitis test (CMT), qualitative chloride percentage and somatic cell count (SCC) were carried out to detect subclinical mastitic cases represented 43.3% and 47.4% respectively. Mastitic cases represented 41.3% and 48.7% of examined ewes and nannies respectively. The isolated organisms were Staph. Aureus, Staph. Epidermidis, Strept. Dysagalactiae, Strept, agalactiae. Strept. Iberis, Past. Haemolytica, Actinomycies ovis, A. pyogens, E. coli and Fungi. Antibiotic sensitivity tests showed variable potency between highly, moderately effective and resistant. In conclusion, this most probably is the first microbiological study for mastitis in small ruminants in the Nomadic area (Haliab, Shalateen and Abu-Ramad). Mastitis is a serious problem in the study area and mastitis control programme is a must to protect Bedouin income and health.