Immunogenicity of an experimentally prepared combined oil emulsified Esherichia coli- Pasteurella multocida (E.coli-P. multocida) vaccine was evaluated in susceptible chickens. The immune responses of vaccinated birds against monovalent E. coli, P. multocida and combined E.coli-P. multocida vaccines as estimated serologically using inderict haemagglutination test and ELISA test revealed no substantial differences with respect to the protective values between the monovalent and combined vaccines. The results of challnge test showed that vaccinated chicken could be effectively immunized with combined E.coli-P. multocida against challenge with E. coli and P. multocida vitulent starins. In conclusion, this  locally prepaed vacine was safe, immunogenic and protect chickens against E. coli and P. multocida infections.