Inactivated tissue culture BF virus vaccine was prepared from the 8^th passage from a locally isolated BEF virus from native breed cattle during BEF outbreak in 2000 in Middle Egypt. The virus was inactivated using Binary ethylene amine (BEI) at concentration of 1% for 3.5 hours. The adjuvant contained mixture of Negilla Sativa oil, mineral oil and vitamin E and selenium with different ratio in two different formula (A-vaccine and B-vaccine). The prepared vaccines were characterized using different techniques including, the drop test, emulsion stability and viscosity. In addition, its provide to.be sterile and safe. These vaccines were compared with aluminum hydroxide gel inactive BEF virus vaccine. B-vaccine induce immune response in vaccinated cattle more than A-vaccine and aluminum hydroxide gel BEF vaccine, while control non-vaccinated groups did not develop any immune response against BEF virus when measure by SPA, VNT and ELISA. Exposure of vaccinated cattle to natural infection with BEF virus revealed no clinical signs of BEF syndrome, while control non-vaccinated cattle groups developed clinical manifestation of BEF syndrome.