The challenge virus standard (CVS-11) strain of fixed rabies virus was propagated on suckling mouse brains for several passages. These passages were titrated in 3-4 weeks old white Swiss mice using mouse inoculation test (MIT). The harvested infected brains of suckling mice were inactivated using 1/4000 beta-propiolactone (BPL) and adjuvanted using Nigella sativa oil and combination of vitamin E& Selenium. The prepared plain and adjuvanted rabies suckling mouse brain vaccines were subjected to quality control tests including its safety, sterility and potency using either National institute of Health (NIH) or immunogenicity test in mice. The obtained results were discussed and revealed that the adjuvanted mouse brain rabies vaccine with either nigella sativa or vitamin E and Selenium were highly immunogenic than the plain vaccine and can be used in human or animals safely.