Plasmid profile analysis of 10 multiple drug-resistant Salmonella serovars with (resistant to ampicillin, chloramphenicol, oxytetracycline, nalidixic acid and streptomycin) was performed using agarose gel electrophoresis. There were 8 isolates harboring plasmid with molecular weight ranged from 16-31.5kpb while the plasmid of 2 isolates could not be detected. Successful transformation of resistant plasmid was done between S.Kentucky and S.Typhimurium as donor bacteria and E.coli XLl as recipient bacteria by electroporation. Transconjugants were obtained by conjugation assay between multiple drug resistant (MDR) S.Typhimurium as donor bacteria and each of S.Entritidis, S.Typhi, E.coli: XLl and E.coli:O157H7 as recipient bacteria.