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379787

ONE-STEP RT-PCR TRAGETING THE FUSION PROTEIN GENE FOR DETECTION OF BOVINE RESPIRATORY SYNCYTIAL VIRUS

Article

Last updated: 03 Jan 2025

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Subjects

-

Tags

Virology and viral diseases

Abstract

RT-PCR F targeting a fragment of the fusion protein (F) gene of bovine respiratory syncytial virus (BRSV) has been recommended to detect BRSV. This assay is used to be performed in two steps after RNA extraction: cDNA synthesis then PCR. In this study, cDNA synthesis and PCR have been performed in one step single tube. This modification may save time and effort with the same sensitivity and specificity.

DOI

10.21608/vmjg.2006.379787

Authors

First Name

NASSER

Last Name

ELERAKY

MiddleName

-

Affiliation

Department of Virology, Faculty of Veterinary Medicine, Sadat City, Menoufia University

Email

-

City

-

Orcid

-

First Name

STEPHEN

Last Name

KANIA

MiddleName

-

Affiliation

Department of Comparative Medicine, College of Veterinary Medicine, University of Tennessee, Konxville, Tennessee, USA.

Email

-

City

-

Orcid

-

First Name

LEON

Last Name

POTGIETER

MiddleName

-

Affiliation

Department of Comparative Medicine, College of Veterinary Medicine, University of Tennessee, Konxville, Tennessee, USA.

Email

-

City

-

Orcid

-

Volume

54

Article Issue

3

Related Issue

50355

Issue Date

2006-07-01

Receive Date

2024-09-13

Publish Date

2006-07-01

Page Start

685

Page End

690

Print ISSN

1110-1423

Online ISSN

2537-1045

Article File

VMJG_Volume 54_Issue 3_Pages 685-690.pdf

PDF . 1.4MB

Link

https://vmjg.journals.ekb.eg/article_379787.html

Detail API

https://vmjg.journals.ekb.eg/service?article_code=379787

Order

379,787

Type

Original Article

Type Code

544

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

ONE-STEP RT-PCR TRAGETING THE FUSION PROTEIN GENE FOR DETECTION OF BOVINE RESPIRATORY SYNCYTIAL VIRUS

Details

Type

Article

Created At

23 Dec 2024