This study was carried out on 72 zoonotic bacteria isolated from 115 milk samples collected from buffaloes with clinical mastitis (43 staphylococcus aureus, 3 Streptococcus agalactiae, 14 E.coil, 6 Klebsiella pneumoniae, 4 Salmonella Typhimurium and 2 Serratia marescens). Antibiotic sensitivity test was done for all zoonotic bacterial isolates. Multiresistant isolates constituted 88.88%. Multiresistance was more among Gram -ve isolates 26/26 (100%) in comparison to Gram +ve isolates 38/46 (82.60%) (P<0.01). plasmids were extracted from 49 out of 64 multiresistant isolated (76.56%). The majority of the isolates contained more than one plasmid. Plasmids were isolated from all Gram -ve isolates (100%) and from 60.52% of Gram +ve isolates (P<0.01). twenty one out of the 26 (80.70%) multiresistant Gram -ve isolates could transfer R-plasmids to sensitive E.coli K12 conjugation. Controlled and proper use of veterinary antibiotics with continuous monitoring of milk for zoonotic multiresistant organisms are recommended to protect human from developing diseases that will not respond to treatment.