In the present study, 250 swabs (174 nasal and 76 ocular) and 510 serum samples were collected from different farms at Kafr el-Sheikh Governorate from calves, feed lot and adult cattle of different ages for PI-3 isolation, identification and seroprevalence. Some of the animals were suffering from the disease. The results indicated that 15 and 12 samples identified as p13 positive by FAT and 12 VNT, respectively. The serodiagnosis results of 510 examined serum samples by HI and SNT revealed that the PI3 antibodies were detected in diseased animals at percentages of 32% and 23.4%, respectively. While in apparently health animals in percentage of 31% and 13.1%, respectively.