In this study, inactivated oil FMD vaccine adjuvanted with ISA 206 was prepared. Guinea pig potency testing was applied to determine the ultimate shelf life of oil adjuvanted FMD vaccine at different conditions of storage temperatures. Our results revealed that guinea pig potency of oil FMD vaccine stored at 4 C was 40.7, 40.7, 31.6, 20.20, 15.8, 10.47, 1.58 at 0, 3, 6, 12, 15, 18, 21, 24 months respectively. While if the vaccine bottle was left at room temperature its guinea pig potency estimates was 40.7, 40.7, 20, 15.8, 1.99 at 0, 1, 2, 3, 4 days respectively. From the previous results, it was found that the ultimate shelf life of oil FMD vaccine is not less than 18 months at 4 C. while if the vaccine bottle is left at room temperature its shelf will be markedly decreased to not more than 3 days. So FMD vaccine must be propely shipped, stored and handled at 4 C. In this study, inactivated oil FMD vaccine adjuvanted with ISA 206 was prepared. Guinea pig potency testing was applied to determine the ultimate shelf life of oil adjuvanted FMD vaccine at different conditions of storage temperatures. Our results revealed that guinea pig potency of oil FMD vaccine stored at 4 C was 40.7, 40.7, 31.6, 20.20, 15.8, 10.47, 1.58 at 0, 3, 6, 12, 15, 18, 21, 24 months respectively. While if the vaccine bottle was left at room temperature its guinea pig potency estimates was 40.7, 40.7, 20, 15.8, 1.99 at 0, 1, 2, 3, 4 days respectively. From the previous results, it was found that the ultimate shelf life of oil FMD vaccine is not less than 18 months at 4 C. while if the vaccine bottle is left at room temperature its shelf will be markedly decreased to not more than 3 days. So FMD vaccine must be propely shipped, stored and handled at 4 C.