Immunogenicity of combined Turkey RhinoTrachitis Virus (TRTV) and Pasterella multocida inactivated oil emulsion vaccine were evaluated in comparison to both the monovalent (TRT) and (P.Multocida) vaccines. Sixty turkeys poults were divided into four groups, group 1, 2, and 3 were vaccinated with (TRTV), pasterella multocida and combined (TRTV + pasterellla multocida) vaccines respectively while group 4 was kept as non vaccinated control, obtained results of cellular (Detected by lymphocyte blastogenesis assay) and Humoral (Detected by indirect haemagglutination IHA, Enzyme Linked Immune Sorbent Assay ELISA, and Serum neutralization test S.N.T) immune response of experimentally vaccinated poults revealed that 2 successive doses of the prepared Vaccine with 21 days apart produce protective immune response started at 3 weeks post vaccination and lasted for 12 weeks covering the whole fattening period.