Differences were noted in the serologic and immunologic responses of chicken and turkeys to vaccination by living avirulent CU fowl cholera vaccine, administrated by wing web or drinking water routes. Turkeys responded to vaccination by the two routes as they developed good humoral serologic response as revealed by IHA and ELISA tests. Local IgA antibodies were detected in tissue sections of lung and intestine of turkeys vaccinated via drinking water route and the protection was 87.5% IgA was detected only in lung tissue section of turkey vaccinated by wing web route. In case of chicken vaccinated by drinking water route, a weak local and humoral response was noted and the protection was 50%. Meanwhile, in chicken vaccinated by the wing web route, a good humoral and local response was observed and 87.5% protection was noted. Inconclusion, turkeys responded to vaccination with avirulent P.multiocida CU strain either via drinking water or wing web routes, while chicken respond to vaccination by the wing web route only but not by the oral route.