One hundred fifty samples were collected from slaughtered sheep and examined for detection of Estradiol 17-Beta residues; 30 samples from each muscle, liver, kidneys, spleen and heart from Basatin, Giza and Beni-suef abattoirs. Collected samples were subjected to the diagnostic kits of Radio Immune Assay (RIA). It was found that samples obtained from El-Basatin abattoir were lower in hormonal residues than those of Giza and Beni-suef, which they were nearly similar to each other. The concentration of hormonal residues was going in descending manner in liver, kidney, spleen, heart and muscle respectively. All results were found to be within the permissible limit. Muscle samples proved to be positive for Estradiol 17-Beta residues were subjected to grilling and boiling for 30 minutes. Boiling was significantly reduced the estradiol 17-Beta residues in muscle at p<0.05. Grilling has no effect on the hormone. The public health significance of estradiol residues was discussed.