Toxoplasmosis and brucellosis are widely distributed, zoonotic diseases affecting a wide spectrum of animals and humans. Serum samples were collected from 182 sheep, 294 goats and 60 human from different localities of Sharkia province (Zagazig - Abo-Hammad - Fakous and Abo-Kabeer) in the period between March - July 2005 and serologically tested for presence of specific antibodies against toxoplasmosis and brucella species. Out of 182 sheep, 48 (26.4%) were sero-positive for toxoplasmosis. By using of enzyme linked immunosorbent assay (ELISA), 17 (9.3%) were IgG, positive and 6 (3.3 %) were IgM positive. Among! goats, 38 (12.93%) were positive by IHAT; and by ELISA, 12 (4.08%) were positive for presence of IgG as well as 9 (3.06%) were positive for presence of IgM. Rose Bengal Plate Antigen (RBPA), Buffered Acidified Plate Antigen (BAPA), Rivanol test (Riv.T) and serum agglutination Test (SAT) were applied to identify brucellosis. Out of 182 sheep sera samples, 21 (11.54%) were sero-positive; Meanwhile out of 294 goat sera, 12 (4.08%) showed sero-posilivity to brucellosis. Brucella melitensis biovar - 3 was isolated from milk samples and tissue specimens (especially lymph nodes) of the reactor animals and from 17 ewes sero-positive to brucellosis there were positive for isolation and identification of Brucella meltiensis, whereas there were MI5 Sero-positive goats (20%) so that control measures of toxoplasmosis and brucellosis were suggested. Concerning to human the incidence of toxoplasmosis and brucellosis were 28.3% and 8.3 % respectively.