Beta
379736

FREEZABILITY OF CAMEL, SEMEN COLLECTED BY “EL-HASSANEIN CAMEL DUMMY” AND DILUTED IN TWO STEPS IN SUCROSE AND / OR TRIS-BASED EXTENDERS

Article

Last updated: 03 Jan 2025

Subjects

-

Tags

Theriogenology / reproductive technology

Abstract

Eight adult male camels were used for semen collection during rutting season (Nov.2001-March,2002). By using the recently invented “El-Hassanein Camel Dummy" at Maryout Research Station of Desert Research Center. Sucrose and Tris and their combinations were used for preparing five extenders for camel semen. Dilution to 150x106 motile sperms/ml was carried out in two steps. Diluted semen filled in 0.5ml straws. Three freezing and two thawing rates were applied to evaluate spermatozoa freezability and post-thawing viability. Progressive motility (PPM) and acrosomal integrity (PIA) were evaluated post-dilution and at 0, 2 and 4 hours of post-thawing incubation. The new collection technique significantly improved the quantity and quality of the delivered semen. The obtained semen volume, ejaculate concentration, progressive motility and acrosomal integrity averaged 15.3 + 1.15 ml, 810.3 +2.21x106 sperm/ml, 81.6 + 1.01% and 89.7+ 1.94%, respectively. Dilution of camel spermatozoa in a Sucrose-Tris extender significantly reduced dilution effect on spermatozoa viability and improved their freezability and their post-thawing viability. Slow and rapid-thawing rates had a relatively comparable effect on post-thawing viability. However, the optimum viability was achieved in semen frozen rapidly (at -140°C for 15 min.) and thawed slowly (at 40°C for 3D sec.) after dilution in an extender composed of: 4.38% (g/v) sucrose 1.592% (g/v) Tris, 0.872 % (g/v) citric acid monohydrate, 20 % (v/v) egg-yolk, 3.5 % (v/v) glycerol, 1000 iu penicillin/ml and 1000 μg streptomycin vial. Estimated reductions in PPM pre-freezing, post-thawing and after 2 and 4 hours of incubation post-thawing were 5.5, 6.1, 23.3 and 42.5%, respectively. Corresponding reductions in PIA were 5.9, 9.1, 15.7 and 35.3%, respectively.  Consequently, it is concluded that combination of sucrose and Tris in camel semen extender has positive effect on viability of spermatozoa after dilution, freezing and post-thawing incubation.

DOI

10.21608/vmjg.2006.379736

Keywords

camel: reproduction, Semen, collection, Dilution, cryopreservation, motility, acrosomal Integrity, freezability, post-thaw survival

Authors

First Name

E

Last Name

EL-HASSANEIN

MiddleName

-

Affiliation

Animal and Poultry Production Division, Desert Research Center, Cairo, Egypt.

Email

-

City

-

Orcid

-

Volume

54

Article Issue

1

Related Issue

50353

Issue Date

2006-01-01

Receive Date

2024-09-13

Publish Date

2006-01-01

Page Start

29

Page End

46

Print ISSN

1110-1423

Online ISSN

2537-1045

Link

https://vmjg.journals.ekb.eg/article_379736.html

Detail API

https://vmjg.journals.ekb.eg/service?article_code=379736

Order

379,736

Type

Original Article

Type Code

544

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

FREEZABILITY OF CAMEL, SEMEN COLLECTED BY “EL-HASSANEIN CAMEL DUMMY” AND DILUTED IN TWO STEPS IN SUCROSE AND / OR TRIS-BASED EXTENDERS

Details

Type

Article

Created At

23 Dec 2024