This study was done on 728 intestinal and liver samples collected from 84 commercial broiler chicken farms aged 1-11 weeks-old located at Behera, Kafr El-Sheikh and El-Gharbia governorates. Anaerobic bacteriological examination revealed isolation of 430 Clostridial strains that identified as; 236 (54.9%) C.perfringens and 194 (45.1%) other clostridial species. Out of the 236 isolated C.perfringens; 61 isolates were toxigenic that subtyped into types A (83.6%), В (1.6%), С (1.6%), and D (13.1%). C.perfringens also re- covered from waterers, feeders and litter sur- rounding the birds with a percentage of 27.3%, 50% and 22.7%; respectively. The mortality rate reached 100% in 5 day-old broiler chicks when intramuscularly inoculated with broth culture of C.perfringens type A,D or b and C toxins of C.perfringens in an arrangement identical to type A,B,C and D. These results suggested that the multiplex-PCR as a rapid, accurate and humanitarian assay could replace the seroneutralization methods with mice and guinea pigs in detection and typing of toxigenic C.perfringens strains.