Bovine theileriosis, babesiosis and anaplasmosis are a haemoparasitic disease caused by protozoans of Genus Theileria, Babesia and Anaplasma respectively. These haemoparasites are responsible for considerable losses due to mortality, weight loss and a reduction in milk beside the cost of prophylactic measures. The results showed that 51 (11.7%) and 13(2.9%) out of 443 cattle from different localities in Tripoli- Libya by Giemsa stained blood and lymph node smears were infected only with Theileria species respectively. The incidence of Theileria species by Giemsa stained blood films examination in different localities in Tripoli was 0.0%, 22.7% and 35.4% in Engeela, Algyran and Fum-Mulgha respectively. While the total incidence of T.mutans, B.bigemina and A.marginale in the examined cattle by different ELISA kits were 50.6%, 12.9, and 3.4%, respectively. Also, the present work recorded mixed infection.