After elapsing of 10 years on the last recorded cases of Foot and Mouth disease (FMD) caused by the virus of serotype A in Saudi Arabia in 1995, new cases of FMD virus, serotype A were diagnosed in two traditional cattle herds in Al- Hota province, 150 Km south of Riyadh. Clinical signs of fever, vesicular stomatitis and coronitis, with morbidity rate of approximately 90% were reported. FMDV, serotype (A) isolates were identified by indirect sandwich ELISA. Vaccine matching tests (virus neutralization and ELISA), amplification and sequencing of the VP 1 of the isolates revealed that the isolates were antigenically closely related with A Saudi 95, A 4164 and A22 Iraq 24/64 strains, and genetically identical with isolates of A Iran 2005. Fortunately one of these strains (A22 Iraq 24/64) is already incorporated in polyvalent FMD-vaccine formula in Saudi Arabia.