A total of 70 vaginal swabs were collected from cows (forty five from cows suffering from congestion and purulent vaginal discharge, and 25 from apparently healthy cows). Also ten vaginal swabs were collected from buffaloes suffering from congestion and purulent vaginal discharge. The collected vaginal swabs were subjected to bacteriological examination for Mycoplasma pathogens and the recovered species were identified using PCR. Vaginal swabs from apparently healthy cows were negative for Mycoplasma isolation, while seven isolate were recovered from vaginal swabs of diseased cows with an incidence of 15.6 %. On the other hand, five isolates were recovered from the vaginal swabs of diseased buffaloes with an incidence of 50%. All Mycoplasma isolates were identified by PCR using M. bovis, M. bovigenitalium, and M. bovine group 7 specific primers. No isolates reacted with M. bovine group 7, while 2 and 6 isolates had amplified fragments at 442 and 928 bp against M.bovis, M.bovigenitalium, respectively. The obtained data indicated that M. bovis was recovered from vaginal swabs of cows and buffaloes with an inci- dence of 2.2 % and 10%, respectively, while the incidence of M. bovigenitalium was 11.1% and 10%, respectively. Ureaplasma could be identified from the collected samples of cows and buffaloes with an incidence of 2.2% and 20%, respectively. Furthermore, another Mycoplasma isolate (unidentified) was recovered from buffalo samples.