Two hundred samples of caseated lymph nodes were collected aseptically from 167 cattle during routine meat inspection at Omdurman central abattoir. The samples were taken on suspecious of Bovine tuberculosis and were subjected to bacteriological investigations. The age of cattle , sites and locations of the caseated lymph nodes were determined during postmortem examination . Mycobacterium bovis was isolated from 11 (5.5 Go) of the caseated lymph nodes . The frequency distribution and locations of isolates of M.bovis includes 6 from the bronchial lymph nodes, 2 from the mediastinal lymph nodes and 3 from the retropharyngeal lymph nodes. With regard to the age of cattle; all isolates of M.bovis were from caseated lymph nodes of old cattle. Other Mycobacterium species involved in nodes includes 18 (9%) M.farcinogenes, 3 (1.5 %) M.avium, 2 (1 %) caseation of lymph M.fortuitum and 2 (1%) M.pheli.Isolation of Mycobacterium species is important in differential diagnosis of Bovine tuberculosis which is a significant zoonotic disease that can transmitted to humans by contamination of meat from infected animals and through aerosols .The role of meat inspection in control of Bovine tuberculosis is discussed .