This study documents an outbreak of scrapie in adult sheep (East — Friesian Breed) in Palestine with high morbidity rate 21.5% . The clinical findings of the disease were abnormal behavior, ataxia, tremor, incoordination of locomotion, pruritus, loss of wool, nibbling, recumbency and hyperaesthesia to noise, movement or touch. The clinical diagnosis is supported by histopathological lesions and immuno-detection of prion proteins in central nervous system by immunohistochemistry using a reference specific staining monoclonal antibody RIDA Mab L42.