In the present study, Fifteen selected probiotic strains of lactic acid bacteria as (lactobacillus, Streptococcus and Bifidobacterium spp) were ex   amined for their ability to remove cholesterol (T-Ch) and triglycerides (TG) from laboratory media during their growth. All selected strains could remove T-Ch and triglycerides (TG) on different media as MRS-THIO broth media GM-THIO growth media except 2 strains only. A combination between the probiotic strains used was carried out to select the proper mixture in removing lipids. Derived yoghurt from the best mixture was analysed for T-Ch and TG under the manufacture conditions proved the ability of chosen mixture in removing lipids. The study extended to evaluate, the effect of yoghurt manufactured by probiotic strains (1% starter ) on the lipid profile through experimental hyperlipidemic rats (rats fed on 1% cholesterol and 0.5% of cholic acid dissolved in 20% coco-nut oil ration for 45 days). The hyperlipidemic rats were subdivided into four groups; Gl, fed on ration free probiotics served as (-) ve control group, G2, 3, 4 fed on ration containing different groups of strains from probiotics, L.acidophillus N10, Str.cremoris F 99 and Bif.ther-bifidum99 (B group); L.acidophillus N10, L.bulgaricus N5 and Str.thermophillus S1 (H group) ; L.acidophillus N10, Str.cremoris F50, L.bulgaricus N5 and Biflongum K69 (E group). The data showed that all the tested groups have similar significant reduction in serum T-Ch, LDL-Ch and TG as well as significant increase in the level of HDL-Ch . All the tested yoghurt showed significant reduction in serum total fatty acids (TFA). The most reduction effection fatty acids were obtained by the group H which could restore the ratio of unsaturated fatty