There are different types of Avian Influenza vaccines were adopted in Egypt after emergence of highly pathogenic Avian Influenza (HPAI) H5N1 in Mid-February 2006 as a tool for control of the disease. These vaccines were used to vaccinate the broiler chickens mostly at one day old. This study investigated the post-vaccinal antibody titer in broiler chickens vaccinated at one day old either by H5NI1 reverse genetic vaccine or HSN2 inactivated vaccine. Hemagglutination inhibition (HI) test was done by using homologous and heterologous antigens. Also, the results of HI test were compared with the Enzyme-linked Immuno- sorbent Assay (ELISA) for detection of antibodies against avian influenza. The results revealed statistical significance differences between the two antigens used in HI test and medium correlation was recorded between HI test and ELISA results. This study revealed that it is highly recommended to use homologous HI antigen in HI test for estimation of Avian Influenza antibodies to accurately evaluate the flock immune status and potency of vaccine used.