The aim of this study was to investigate piscine mycobacteriosis in wild sharptooth catfish, Clarias gariepinus. Out of 120 fish collected, Mycobacterium SPP. Were isolated from fish 5 (4.16%),  M. fortuitum was isolated from 3 (2.5%), while M. marinum was isolated from 2 (1.67%) fish. methods were applied to identify suspected Conventional and molecular mycobacterial isolates. Experimental induction of mycobacteriosis in sharptooth catfish by intraperitoneal inoculation and 1.2X10⁸ and 1.6X10⁸ cfu of M. fortuitum (MF4) or M. marinum (MM31), respectively, resulted in acute infections with severe peritonitis and adhesions. Less severe to chronic cases resulted from intraperitoneal inoculation of 1.2X10³' and 1.6X10⁷ cfu of M. fortuitum and M. marinum, respectively. Sharptooth catfish with induced chronic M. fortuitum infections showed severe enlargement of the spleen and dark coloration of the liver and kidneys, while chronic M. marinum Showed sanguineous granular ascites. Antibiograms of The fisherman dealing with sharptooth catfish had induced marinumm the isolates were also conducted. developed nodules on the dorsum of hand that could be a case of fish handler granuloma.