Biotyping of 9 isolates of Cryptococcus neoformans recovered from bird droppings was carried out on Canavanine-glycine-bromothymol blue (CGB) and Glycine-cycloheximide-phenol red (GCP) media. Only one isolate was identified as C. neoformans var gattii (serotype B-C) because it produced cobalt-blue colour on CGB medium and red colour on GCP medium. Five isolates were considered as C. neoformans var neoformans (serotype A-D) as they produced no change in colour on both media, while 3 isolates were suspected to be C. neoformans var neoformans, as they produced light blue colour on CGB medium, but no colour on the GCP medium. Polymerase chain reaction (PCR) was used for identifying and biotyping of six C. neoformans isolates where two sets of base pairs C. neoformans biotypes specific were applied. The PCR results revealed that 5-isolates, which were identified and biotyped by conventional methods as C. neoformans var neoformans, in addition to the standard strain responded to the first primer of 695 bp, i.e they were identified as serotype A. Only one isolate, which was identified as C. neoformans vagattii was amplified by the second primer pair 448 bp that confirmed this isolate as serotype B.