The present study was carried out on 48 brucella positive- slaughtered cows, 37 animals from the Experimental Station belonging to the Faculty of Veterinary Medicine Beni Seuf University and 1 animals from a private farm at Ehnasia, Beni Seuf governorate. The pathological study revealed the presence of individual variations With respect to organs affected, extension and severity of lesions. Granulomatous reactions were detected in lymphoreticular tissues and also in the liver, kidneys, lungs and udder. Epithelioid cell and giant cell granulomas were predominating in the lymph nodes and liver and tuberculous-like granulomas were found in the kidneys and lung. By immunostaining, Brucella melitensis could be detected in organs and lymph nodes.