This study was performed to compare the of oesophogeal muscles with that of Enzyme- linked assay (ELISA) for detecting Sarcocystis infection in cattle. For result of microscopical examination Immunosorbent this purpose, one hundred and twenty samples of muscles and blood were collected and examined. Sarcocystis cysts were detected Microscopically in 81.6 % of the examined cattle, while, by ELISA with two prepared antigens of bradyzoites from cattle Sarcocystis spp. and S. fusiformis of buffaloes detected specific antibodies in 83% and 85% of the tested serum samples, respectively. The sensitivity of both antigens was 100%, while the specificity was 91% and 82% for bradyzoites of cattle Sarcocystis SPP. Antigen and S. fusiformis antigen, respectively. Rabbit hyper-immune sera raised against bradyzoites of cattle Sarcocystis spp. antigen reacted against 2 bands with M.W. 26KDa and 28.5KDa in both antigens. One band with M.W. 31 KDa was recognized with bradyzoites of cattle Sarcocystis spp. antigen. ELISA using bradyzoites of cattle Sarcocystis spp. Antigen was found to be suitable and can be utilized for diagnosis Sarcocystis infections in cattle