The clinical, radiographic, and  histological effect of chitosan on the healing process of experimentally induced central frog deep wounds as well as the accompanied hematological changes were studied in 6 female adult donkeys. All operated lesions healed satisfactory without any clinical or radiographic signs of infection. The grade of lameness was greatly improved and the regenerated soft tissues filled the frog wound within one week after chitosan treatment. Thin covering layer of horny tissues formed two weeks later while the whole wound closed within eight weeks after chitosan treatment. The hematological profile revealed that the coagulation parameters after local chitosan application greatly enhanced hemostasis through striking increase of platelets count which were reflected on the marked reduction of PT and APPT in all tested donkeys post- operatively in comparison with pre-operative standards. Generally, no considerable differences were recorded between pre-operative and days post-operative (PO).up to 14 days for the values of hemogram (Hb, RBC,, PCV and blood indices), fibrinogen, total protein and glucose. The obtaifed levels of ALT and AST at 3 days PO showed a significant elevation and returned to the preoperative levels after 7 days PO Histological examination proved the efficacy) of the regenerative power of chitosan as both the epidermal and dermal layers showed newly formed capillaries with acanthosis and polyp formation in the prickle cell layer of the epidermis and connective tissue core formation in the dermis. From this study, it can conclude that chitosan is an excellent dry dressing