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380149

TYPING OF STREPTOCOCCI BY MEANS OF SPECIFIC ANTIBODY ADSORBED TO PROTEIN-A CONTAINING STAPHYLOCOCCI

Article

Last updated: 03 Jan 2025

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Subjects

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Tags

Bacteriology and bacterial diseases

Abstract

Staphylococcus protein-A (SPA) is a cell wall consti- tuent of Staphylococcus aureus which has an extra - ordinary affinity for binding to the FC region of immunoglobulin G (IgG) from various mammalian species. Cell bound SPA is found to be useful as an adsorbent for detection of antigen-antibody complex (Goding, 1978, Kessler, 1981 and Lindmark et al., 1983). The precipitation test is widely used for typing of - streptococci by means of their carbohydrate substance or antigen "C" in their cell walls (Crusickshank et al., 1975). This test however, more laborious and time consuming as a routine test. A slide agglutina- tion method is here described, that renders strepto- coccal typing quick and easy to be performed. when specific anti-streptococcal antibodies, were added to a stabilized suspension of staphylococci , they  became bound to the protein A on the cell wall via   the FC structure of IgG (Kronvall and Williams ,1969 and Kronvall and Frommel, 1970); therefor ,orienta- ting the Fab-located antibody- combining sites outwards  (Lind et al., 1972). When streptococci  of the corresp onding group are added, coagglutinin occurs with the antibody coated staphylococci. Thus , this work was done to determine the more economic ,rapid and  easy  method for typing of the examined streptococci.

DOI

10.21608/vmjg.1990.380149

Authors

First Name

M.

Last Name

ISMAIL

MiddleName

-

Affiliation

Department of microbiology, Faculty of Veterinary Medicine, Cairo University.

Email

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City

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Orcid

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Volume

38

Article Issue

1

Related Issue

50230

Issue Date

1990-01-01

Receive Date

2024-09-17

Publish Date

1990-01-01

Page Start

45

Page End

51

Print ISSN

1110-1423

Online ISSN

2537-1045

Article File

VMJG_Volume 38_Issue 1_Pages 45-51.pdf

PDF . 3.6MB

Link

https://vmjg.journals.ekb.eg/article_380149.html

Detail API

https://vmjg.journals.ekb.eg/service?article_code=380149

Order

380,149

Type

Original Article

Type Code

544

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

TYPING OF STREPTOCOCCI BY MEANS OF SPECIFIC ANTIBODY ADSORBED TO PROTEIN-A CONTAINING STAPHYLOCOCCI

Details

Type

Article

Created At

23 Dec 2024