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379225

THE VALIDITY OF ENZYME LINKED IMMUNOSORBANT ASSAY IN DETECTION OF SALMONELLA IN DRESSED POULTRY

Article

Last updated: 23 Dec 2024

Subjects

-

Tags

Avian diseases / production
Bacteriology and bacterial diseases
Immunology / vaccine / diagnosis

Abstract

Salmonellae which are endemic in many poultry flocks, may causing minimal poultry health problems. However during the slaughter of such poultry by assembly-line procedures encourages cross contamination particularly in the slaughter operation with continuous water immersion chilling often takes place This fact explains why the poultry industry has received most of the blame for contamination of the food chain with salmonellae. Traditional methods for the isolation of salmonellae from poultry which involve non-selective pre-enrichment followed by secondary enrichment in selective broth media and subculture onto selective differential agars, have been used by many authors, (Wassef et al., 1980; El-Mossalami et al., 1986 and Nouman et al., 1986). Such methods are both labour, intensive and time consuming while a sample can provisionally be declared salmonella positive within 3 days, a final answer on a negative sample can take up to 5 days. Therefore a wide interest in a more rapid methods for the detection of salmonella and a number of procedures of varying complexity have been put forward. These include; the use of immunofluorescent technique (Safwat et al., 1981) Enrichment serology, radiometry, enzyme immunoassays and the used of gene probes (Emswiler et al., 1984; Rigby 1984; and Todd et al., 1986). The trend in this study is to verify the suitability of the enzyme immunoassay as rapid test for the detection of salmonellae in dressed broilers.

DOI

10.21608/vmjg.1991.379225

Authors

First Name

T.

Last Name

NOUMAN

MiddleName

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Affiliation

Faculty of Vet. Med. Cairo University

Email

-

City

-

Orcid

-

First Name

S.

Last Name

SAFWAT

MiddleName

-

Affiliation

Animal Health Research Institute-Dokki, Cairo

Email

-

City

-

Orcid

-

First Name

A.

Last Name

DARWISH

MiddleName

-

Affiliation

Faculty of Vet. Med. Cairo University

Email

-

City

-

Orcid

-

First Name

M.

Last Name

EL-SHATER

MiddleName

-

Affiliation

Animal Health Research Institute-Dokki, Cairo

Email

-

City

-

Orcid

-

Volume

39

Article Issue

3

Related Issue

50228

Issue Date

1991-07-01

Receive Date

2024-09-10

Publish Date

1991-07-01

Page Start

833

Page End

840

Print ISSN

1110-1423

Online ISSN

2537-1045

Link

https://vmjg.journals.ekb.eg/article_379225.html

Detail API

https://vmjg.journals.ekb.eg/service?article_code=379225

Order

379,225

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

THE VALIDITY OF ENZYME LINKED IMMUNOSORBANT ASSAY IN DETECTION OF SALMONELLA IN DRESSED POULTRY

Details

Type

Article

Created At

23 Dec 2024