Twentry-four Barki male lambs (1.04 + 0.1 months, age) were equally divided into two groups: the first represented the fresh water-treated (0.3 g/1 TDS) group (FW) and the second one used as saline-treated (13.1 2/1 TDS) group (SW). The experiment extended for about 22 months. During the last two months, animals trained for semen collection (using artificial vagina), then, semen quality of the first and second ejaculates, for nine rams from each group, was evaluated at 3-day intervals. A Long-term saline treated rams showed highly significant reduction (P < 0.01) in the sperm-cells concentration and the percentage of alive sperm-cells and advanced motility of spermatozoa. This was coincided with highly significant increase (P < 0.01) in the percentage of the sperm-cells primary and secondary abnormalities. Biochemical evaluation of semen reflected that SW-treated rams showed a highly significant increase (P < 0.01) in fructose content and a highly significant reduction (P < 0.01) in citric acid concentration and acetylcholinesterase enzyme activity. On the other hand, a highly significant negative correlation was found between fructose content and each of the sperm-cells concentration, percentage of the advances motility and percentage of the alive sperm-cells, however, acetylcholinesterase enzyme activity showed a highly significant (positive correlation with each of the three (physical parameters. The enzyme activity negatively correlated with the percentage of primary and secondary abnormalities of sperm-cells. The experiment showed a marked adverse effect on the semen quality of rams grown on drinking saline water.