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376416

IMMUNE RESPONSES OF IMMUNIZAED BALB/C MICE WITH AVIAN REO VIRUS

Article

Last updated: 03 Jan 2025

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Subjects

-

Tags

Immunology / vaccine / diagnosis

Abstract

  As preliminary procedure for production of monoclonal antibodies against avian reovirus, cellular and humoral immunity were measured for immunized female balb/c mice after continuous exposure to purified avian reovirus 17.33 . Immunoglobulin concentrations of IgG, IgG1, IgG2a, IgG2b, IgG3, IgM, IgA, IgA were measured in sera of mice at different intervals post inoculation with one, two, three , four doses of purified avian reovirus 1733, using Radial immunodiffusion test. At the time antibody titers were measured in sera of immunized mice by enzyme immunosorbent assay using single dilution of sera.    Immunoglobulin secreting cells were counted in spleen of immunized mice, one week post second, third and fourth doses of avian reovirus using solid phase immunoenzymatic technique. The results indicated that there was no relation between hyperimmunization of mice and optimum time to obtain mature, large differentiated specific-B-lymphocyte.  

DOI

10.21608/vmjg.1996.376416

Authors

First Name

AMINA

Last Name

.

MiddleName

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Affiliation

Animal Health Research Institute, Dokki, Cairo, Egypt

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Orcid

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First Name

A.

Last Name

NAWWAR

MiddleName

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Affiliation

Animal Health Research Institute, Dokki, Cairo, Egypt.

Email

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City

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Orcid

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First Name

TH

Last Name

.

MiddleName

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Affiliation

Animal Health Research Institute, Dokki, Cairo, Egypt.

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First Name

ABDEL AZIZ

Last Name

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MiddleName

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Affiliation

Animal Health Research Institute, Dokki, Cairo, Egypt.

Email

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Orcid

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First Name

EL-EBIARY

Last Name

.

MiddleName

-

Affiliation

Animal Health Research Institute, Dokki, Cairo, Egypt.

Email

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City

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Orcid

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First Name

A.

Last Name

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MiddleName

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Affiliation

Animal Health Research Institute, Dokki, Cairo, Egypt.

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First Name

NADA

Last Name

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MiddleName

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Affiliation

Animal Health Research Institute, Dokki, Cairo, Egypt.

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First Name

ILS

Last Name

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MiddleName

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Affiliation

Animal Health Research Institute, Dokki, Cairo, Egypt.

Email

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Orcid

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Volume

44

Article Issue

1

Related Issue

50019

Issue Date

1996-01-01

Receive Date

2024-08-27

Publish Date

1996-01-01

Page Start

41

Page End

47

Print ISSN

1110-1423

Online ISSN

2537-1045

Article File

VMJG_Volume 44_Issue 1_Pages 41-47.pdf

PDF . 6.3MB

Link

https://vmjg.journals.ekb.eg/article_376416.html

Detail API

https://vmjg.journals.ekb.eg/service?article_code=376416

Order

376,416

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

IMMUNE RESPONSES OF IMMUNIZAED BALB/C MICE WITH AVIAN REO VIRUS

Details

Type

Article

Created At

23 Dec 2024