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375664

COMPARISON BETWEEN SOLID PHASE ELISA, DOT ELISA AND AGAR GEL PRECIPITATION TEST FOR DETECTION OF BURSAL DISEASE VIRAL ANTIGEN IN BURSAL HOMOGENATES

Article

Last updated: 23 Dec 2024

Subjects

-

Tags

Virology and viral diseases

Abstract

Forty-four bursal samples were collected from naturally infected and normal broiler chicks. The samples were homogenized and the homogenates were tested by agar gel precipitation test (AGPT), solid-phase ELISA (by indirect double antibodies sandwitch and single antibody ELISA systems) and Dot ELISA for detection of infectious bursal disease virus (IBDv) antigen. The reacted positive samples showed 86.3,88.6 and 88.6 percentage in AGPT, solid-phase ELISA and Dot ELISA, respectively. The findings suggest that the Dot ELISA is faster, more economic and easily applicable procedure than the other two techniques. The indirect double antibody sandwitch technique-is more sensitive than the indirect single antibody EIISA system.

DOI

10.21608/vmjg.1995.375664

Authors

First Name

MADBOULY

Last Name

M

MiddleName

-

Affiliation

Ass. Prof. of virology, Fac. Vet. Med.; Beni-Suef, Cairo Univ.

Email

-

City

-

Orcid

-

First Name

MANAL

Last Name

AFIFI

MiddleName

-

Affiliation

lecaturer of Poultry Dis., Fac. Vet.. Med; Cairo University.

Email

-

City

-

Orcid

-

Volume

43

Article Issue

1

Related Issue

49958

Issue Date

1995-01-01

Receive Date

2024-08-24

Publish Date

1995-01-01

Page Start

37

Page End

42

Print ISSN

1110-1423

Online ISSN

2537-1045

Link

https://vmjg.journals.ekb.eg/article_375664.html

Detail API

https://vmjg.journals.ekb.eg/service?article_code=375664

Order

375,664

Type

Original Article

Type Code

544

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

COMPARISON BETWEEN SOLID PHASE ELISA, DOT ELISA AND AGAR GEL PRECIPITATION TEST FOR DETECTION OF BURSAL DISEASE VIRAL ANTIGEN IN BURSAL HOMOGENATES

Details

Type

Article

Created At

23 Dec 2024