‘The incidence of mycoplasma infections in turkey flocks from different localities of Egypt was surveyed by testing (150) serum samples and (150) samples collected from lungs, air sacs, tracheas and swabs from cloaca, oropharynx, nasal pasages and sinuses of turkeys of different ages. Serum samples were subjected to serological examination using slide agglutination (SA) and mycoplasmas affecting turkeys are M.haemagglutination inhibition (HI) tests. It was found that, out of (150) serum samples 135 (90%) were serologically positive to M. gallisepticum, 50 (33.3%) to M. meleagridis and 20 (13.370) were positive to M. synoviae. Isolation and identification of mycoplasma  revealed the presence of M. gallisepticum in 80 (53.4%) samples, M. meleagridis in 42 (28%), while M. synoviae was isolated from 14 (9.3%) samples, 6 (4%) isolates were not identified by antisera, but their biochemical pattern suggested M. lowae. The in-vitro sensitivity test indicated that, the examined serotypes was highly sensitive to Lincospectin and kitamox and less sensitive to Oxytetracyclin and Doxycycline.