Two isolates of suspected virus are obtained from different broiler flocks 2-3 week in age. These broiler flocks had problems suggestive of chicken infectious anaemia (CIA) such as poor flock performance, poor weight gain, anaemia and increased mortality.
The identification of the virus strains as CIAV is based on
1-The lesions, recorded in naturally infected chickens were, compatible with those reported for CIA (thymus atrophy, yellow bone marrow, anaemia and haemorrhage).
2-These lesions were also reproduced in chickens inoculated with materials treated with chloroform and passed through 50 nm filters. 
3- Low haematocrițe value of naturally and experimentally infected chickens under investigation (27%).
4- Inoculation of embryonated chicken eggs via yolk sac.
5- Isolated virus strains did not agglutinate chicken and/or sheep erythrocytes (RBCs).
6- The isolates induced histopathological lesions which were a reflection of macroscopic observations.
7- These findings are the characteristic lesions of the virus and demonstrate that CIAV is present in some flocks in Sharkia.