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CHROMOSOME CONFIGURATION DURING IN VITRO MATURATION OF DROMEDARY CAMEL OOCYTES

Article

Last updated: 03 Jan 2025

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Subjects

-

Tags

Theriogenology / reproductive technology

Abstract

The aim of the present study was to examine the changes in chromosomal configuration of camel oocyte during in vitro maturation (IVM) at different culture periods of 24, 30, 36 and 48 hours and to compare the influence of pregnant camel serum and fetal calf serum on IVM of camel oocytes 24-30 hours. A total of 1978 oocytes were collected from 390 ovaries of slaughtered camels by aspiration method. The average number of camel oocytes per ovary was 5.30 ± 0.22. The proportion of COC, POC and DO oocytes was 29.27, 32.90 and 32.61% respectively. In addition, 5.21% of oocytes were degenerated. A high percentage of maturation was obtained at 24-30 hours (47.70% and 48.35% respectively). At 48 hours post-maturation, all metaphases were undefined due to degeneration of chromatin.  it was found that, addition of pregnant camel serum had no beneficial effect on IVM of camel oocytes in comparison to fetal calf serum (40.10 vs 45.34% respectively).

DOI

10.21608/vmjg.2003.375375

Authors

First Name

KARIMA GH

Last Name

MAHMOUD

MiddleName

-

Affiliation

Dept. of Animal Reproduction and A.L., National Research Center, Dokki. Egypt

Email

-

City

-

Orcid

-

First Name

K

Last Name

EL-SHAHAT

MiddleName

-

Affiliation

Dept. of Theriogenology, Fac. Vet. Med., Cairo Univ., Egypt

Email

-

City

-

Orcid

-

First Name

W

Last Name

EL-NATTAT

MiddleName

-

Affiliation

Dept. of Animal Reproduction and A.L., National Research Center, Dokki. Egypt

Email

-

City

-

Orcid

-

Volume

51

Article Issue

3

Related Issue

49905

Issue Date

2003-07-01

Receive Date

2024-08-21

Publish Date

2003-07-01

Page Start

411

Page End

420

Print ISSN

1110-1423

Online ISSN

2537-1045

Article File

VMJG_Volume 51_Issue 3_Pages 411-420.pdf

PDF . 2.6MB

Link

https://vmjg.journals.ekb.eg/article_375375.html

Detail API

https://vmjg.journals.ekb.eg/service?article_code=375375

Order

375,375

Type

Original Article

Type Code

544

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

CHROMOSOME CONFIGURATION DURING IN VITRO MATURATION OF DROMEDARY CAMEL OOCYTES

Details

Type

Article

Created At

23 Dec 2024