A total of 40 (20 from each) diseased and apparently healthy ostriches were examined. The age of examined birds ranged between 3-12 months were suffering from respiratory diseases. Samples were collected from private farms in El-Wady El- Gadid and Alexandria Governorates. The most prevalent bacteria were E. coli (53.33%) followed by S. aureus (30%). P. aeruginosa (21.66%), P. haemolytica (20.83%), Micrococcus spp. (18.33%), K. pneumoniae (16.66%), S. pneumoniae (15.83%), P. mirabilis (15%), while A. pyogenes (14.16%), P. multocida and C. freundii (7.50%), other bacterial pathogens isolated in low rates were non haemolytic streptococci, Corynebacterium haemolytica, P. vuglaris and K. oxyto- ca. The most predominant E. coli serogroups were: 01, 06, 018, 025, 026, 044, 055, 078, 086, 0114, 0119, 0125, 0142, 0152, 0157 and five strains were serologically untyped, mean- while serogrouping of P. aeruginosa revealed “9" different “O" serogroups which were (A.B.F.I.H.J.K.L.N.). Whereas antibodies of M. gallisepticum were detected in sera of three dis- eased ostriches. Antibiograms were done on the most predominant isolates. Infected ostriches showed significant increase in some haematological values (MCV, MCH, total leucocytes, lymphocytes and monocytes), but RBCs, PCV and Hb% were de- creased. A marked elevation in serum levels of total protein, globulin, AST, ALT, K, calcium and magnesium were recorded.