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375142

DIRECT DETECTION OF VARIANT INFECTIOUS BURSAL DISEASE VIRUS IN VACCINATED EGYPTIAN BROILER FLOCKS USING ANTIGEN-CAPTURE ELISA

Article

Last updated: 03 Jan 2025

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Subjects

-

Tags

Virology and viral diseases

Abstract

Infectious bursal disease virus (IBDV) serotype I isolates result in serious economic losses to the Worldwide poultry industry. Variant IBDV strains Are usually isolated from flocks vaccinated using classical IBDV vaccines. Monoclonal antibody typing has been used to differentiate between classical and variant strains. A total of 277 bursal samples from 13 vaccinated broiler flocks were Tested for IBDV subtypes. A panel of 4 monoclonal antibodies directed against VP2 epitopes was used to test the bursal homogenates using an antigen-capture ELISA (AC-ELISA). Of the total Number of samples, 73.6% were IBDV-antigen- Positive, of which, 41.7% were classical IBDV And 58.3% were variant IBDV. Del/E was the predominant variant detected in the tested flocks (51.3% of IBDV-positive samples). Moreover, 47% of the total variants were untypeable. The Presence of variant IBDV in vaccinated Egyptian Broiler flocks demonstrates the need for a revision of current control strategies and vaccination Programs.

DOI

10.21608/vmjg.2003.375142

Keywords

IBDV, Classical, Variant, Antigen-Capture, Antigenic profile

Authors

First Name

A

Last Name

METWALLY

MiddleName

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Affiliation

Private Poultry Diagnostic Laboratory, Giza, Egypt.

Email

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City

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Orcid

-

First Name

M

Last Name

SABRY

MiddleName

-

Affiliation

Egytech, Dokki, Cairo, Egypt.

Email

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City

-

Orcid

-

First Name

A

Last Name

SAMY

MiddleName

-

Affiliation

Virology Department, Faculty of Veterinary Medicine, Cairo University, Egypt.

Email

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City

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Orcid

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First Name

M

Last Name

OMAR

MiddleName

-

Affiliation

Private Poultry Diagnostic Laboratory, Giza, Egypt.

Email

-

City

-

Orcid

-

First Name

A

Last Name

YOUSIF

MiddleName

-

Affiliation

Virology Department, Faculty of Veterinary Medicine, Cairo University, Egypt.

Email

-

City

-

Orcid

-

First Name

I

Last Name

REDA

MiddleName

-

Affiliation

Virology Department, Faculty of Veterinary Medicine, Cairo University, Egypt.

Email

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City

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Orcid

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Volume

51

Article Issue

1

Related Issue

49903

Issue Date

2003-01-01

Receive Date

2024-08-21

Publish Date

2003-01-01

Page Start

105

Page End

119

Print ISSN

1110-1423

Online ISSN

2537-1045

Article File

VMJG_Volume 51_Issue 1_Pages 105-119.pdf

PDF . 3.4MB

Link

https://vmjg.journals.ekb.eg/article_375142.html

Detail API

https://vmjg.journals.ekb.eg/service?article_code=375142

Order

375,142

Type

Original Article

Type Code

544

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

DIRECT DETECTION OF VARIANT INFECTIOUS BURSAL DISEASE VIRUS IN VACCINATED EGYPTIAN BROILER FLOCKS USING ANTIGEN-CAPTURE ELISA

Details

Type

Article

Created At

23 Dec 2024