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375140

EXPERIMENTAL EVALUATION OF DIFFERENT VACCINES AGAINST TRICHOPHYTOSIS IN GUINEA PIGS AND CALVES

Article

Last updated: 03 Jan 2025

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Tags

Bacteriology and bacterial diseases

Abstract

Hairs and skin scales from 80 cattle with skin lesions, resembling ringworm were examined mycologically and T. verrucosum was the main common fungus isolated from 3 cases (3.75%). Four types of vaccines were locally prepared, namely living attenuated, formalin-inactivated, Phenol-inactivated and irradiated vaccines. The Later vaccine was produced using a dose of 2KGy Cobalt gamma irradiation, which was sufficient For complete inactivation of T. verrucosum and used for the preparation of the vaccine. Evaluation of the produced vaccines for purity and sterilIty was done. Aqueous whole dermatophyte extract antigen was prepared by mechanical Disruption of dried mycelial powder of T. verrucosum. The ELISA optical density mean values of Anti-T. Verrucosum IgG in sera of calves vaccinated with the four types of vaccines were determined. Marked positive delayed skin reactions Were clearly observed in all vaccinated calves with different types of the prepared vaccines 48h After injection. Living attenuated and phenol inactivated vaccines proved to be the best inducers of allergic response in both calves and guinea pigs and the other two vaccines also sensitized guinea Pigs with smaller degree. On the basis of the average values of the macrophage migration inhibition test in guinea pigs, it Indicated that the highest cellular immune response of guinea pigs after the first and second injection was due to the variant of the prepared vaccine and the reactions were more intensive Following the first dose of the vaccines than after the second injection. Challenge tests in both immunized calves and guinea pigs were discussed in details.

DOI

10.21608/vmjg.2003.375140

Authors

First Name

F

Last Name

EL-SEEDY

MiddleName

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Affiliation

Dept. of Bacteriology, Mycology and Immunology, Fac. Vet. Med. Beni Suef, Cairo Univ.

Email

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City

-

Orcid

-

First Name

I

Last Name

RADWAN

MiddleName

-

Affiliation

Dept. of Bacteriology, Mycology and Immunology, Fac. Vet. Med. Beni Suef, Cairo Univ.

Email

-

City

-

Orcid

-

First Name

HEIDI M

Last Name

SHAWKI

MiddleName

-

Affiliation

Dept. of Microbiology, Fac. Vet. Med., Cairo Univ.

Email

-

City

-

Orcid

-

First Name

W

Last Name

HASSAN

MiddleName

-

Affiliation

Dept. of Bacteriology, Mycology and Immunology, Fac. Vet. Med. Beni Suef, Cairo Univ.

Email

-

City

-

Orcid

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Volume

51

Article Issue

1

Related Issue

49903

Issue Date

2003-01-01

Receive Date

2024-08-21

Publish Date

2003-01-01

Page Start

83

Page End

94

Print ISSN

1110-1423

Online ISSN

2537-1045

Article File

VMJG_Volume 51_Issue 1_Pages 83-94.pdf

PDF . 2.9MB

Link

https://vmjg.journals.ekb.eg/article_375140.html

Detail API

https://vmjg.journals.ekb.eg/service?article_code=375140

Order

375,140

Type

Original Article

Type Code

544

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

EXPERIMENTAL EVALUATION OF DIFFERENT VACCINES AGAINST TRICHOPHYTOSIS IN GUINEA PIGS AND CALVES

Details

Type

Article

Created At

23 Dec 2024