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377265

USE OF WESTERN BLOT TECHNIQUE FOR THE DETECTION OF THE IMMUNOGENIC DETERMINTS OF CLOSTRIDIUM CHAUVOEL WHOLE CELLULAR ANTIGEN

Article

Last updated: 03 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Immunology / vaccine / diagnosis

Abstract

Sodium dodcyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was used for fractionation of the whole cellular antigens of C.chauvoei. The obtained results revealed the presence of 8 polypeptide bands which ranged from 205 to 18.5 KDa. A major protein band with a molecular weight of 49.5 KDa was identified. Application of the immunoblotting technique with the specific hyperimmune serum prepared against C.chauvoei whole cellular formalinized antigen in rabbits, showed the presence of some major antigenic epitopes which reacted strongly with the specific hyperimmune serum, mainly at 29.5 and 205 KDa.

DOI

10.21608/vmjg.1998.377265

Authors

First Name

M.

Last Name

M.EL-BARDISY

MiddleName

-

Affiliation

Animal Health Rescarch Institute, Dokki, Egypt.

Email

-

City

-

Orcid

-

First Name

M.A

Last Name

GABAL

MiddleName

-

Affiliation

Dept. of Microbiology, Immunology and Preventive Medicine, ISU.

Email

-

City

-

Orcid

-

Volume

46

Article Issue

2

Related Issue

49900

Issue Date

1998-04-01

Receive Date

2024-08-30

Publish Date

1998-04-01

Page Start

171

Page End

176

Print ISSN

1110-1423

Online ISSN

2537-1045

Article File

VMJG_Volume 46_Issue 2_Pages 171-176.pdf

PDF . 2.7MB

Link

https://vmjg.journals.ekb.eg/article_377265.html

Detail API

https://vmjg.journals.ekb.eg/service?article_code=377265

Order

377,265

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

USE OF WESTERN BLOT TECHNIQUE FOR THE DETECTION OF THE IMMUNOGENIC DETERMINTS OF CLOSTRIDIUM CHAUVOEL WHOLE CELLULAR ANTIGEN

Details

Type

Article

Created At

23 Dec 2024