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377240

DETECTION OF RINDERPEST VIRUS BY POLYMERASE CHAIN REACTION (PCR) AND SOUTHERN BLOT HYBRIDIZATION

Article

Last updated: 03 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Veterinary medicine / clinical pathology

Abstract

Rinderpest Is an economically highly important disease affecting cattle and buffalo. It's endemic throughout large parts of Africa, Asia, and the Middle East (Egypt). The reverse transcription reaction (RT-PCR), using phosphoprotein (P) gene specific primers set, was used to detect rinderpest virus extracted from infected VERO cells. The amplified 429 bp PCR product was subjected to digestion with restriction endonucleases enzymes (Alu 1 & Hae III) which cut at specific sequence sites derived from rinderpest phosphoprotein (P) gene. The PCR product served as a probe for detection of cDNA derived from infected VERO cells with rinderpest, so PCR assay when combined with southern blot hybridization hopefully will pave the way for rapid detection, monitoring, and surveillance of rinderpest infection.  

DOI

10.21608/vmjg.1998.377240

Authors

First Name

M.S.

Last Name

SABER

MiddleName

-

Affiliation

Faculty of Veterinary Medicine, cairo University, Giza, Egypt

Email

-

City

-

Orcid

-

First Name

A.M.

Last Name

ABBAS

MiddleName

-

Affiliation

Genetic Engineering Unit, Veterinary Serum and Vaccine Research Institute, Abasssia, Cairo, Egypt

Email

-

City

-

Orcid

-

Volume

46

Article Issue

2

Related Issue

49900

Issue Date

1998-04-01

Receive Date

2024-08-29

Publish Date

1998-04-01

Page Start

125

Page End

132

Print ISSN

1110-1423

Online ISSN

2537-1045

Article File

VMJG_Volume 46_Issue 2_Pages 125-132.pdf

PDF . 3.1MB

Link

https://vmjg.journals.ekb.eg/article_377240.html

Detail API

https://vmjg.journals.ekb.eg/service?article_code=377240

Order

377,240

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

DETECTION OF RINDERPEST VIRUS BY POLYMERASE CHAIN REACTION (PCR) AND SOUTHERN BLOT HYBRIDIZATION

Details

Type

Article

Created At

23 Dec 2024