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372275

PREPARATION AND LABORATORY EVALUATION OF LIVE ATTENUATED INFECTIOUS BURSAL DISEASE (IBD) VIRUS VACCINE FROM RECENT EGYPTIAN ISOLATE PROPAGATED ON CHICKEN EMBRYO FIBROBLAST (CEF)

Article

Last updated: 03 Jan 2025

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Subjects

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Tags

Virology and viral diseases

Abstract

Very virulent IBDV was propagated for 3 passages in SPF-ECE followed by 3 passages in young susceptible chicks then for 3 passages in SPF. ECE then for further 12 passages in SPF-ECE. The first passage of last propagation was further propagated for 3 passages on Vero cells and for 60 passages in CEF-cell culture. The passage 40 of propagated IBDV proved to be safe and highly immunogenic by virus neutralization test. It still elicited high neutralizing antibody titers (18 log2) for a stationary phase about 7 months (the end of the experiment) and high values of lymphocyte blastogenesis (0.560 versus control 0.03). Vaccinated chicks well protected against challenge with highly virulent IBDV after 3 weeks post-vaccination (PV). The prepared vaccine has superior potential immunogenic effect than commercial live mild, and intermediate plus (hot) vaccines. The vaccine is effective even when preserved for 8 months (the end of ex- - periment) at -20°C.

DOI

10.21608/vmjg.2001.372275

Authors

First Name

H

Last Name

MADBOULY

MiddleName

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Affiliation

Faculty of Veterinary Medicine, Cairo University, Beni-Suef branch.

Email

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City

-

Orcid

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First Name

ENSAF M

Last Name

KHASHABAH

MiddleName

-

Affiliation

Veterinary Serum and Vaccine Research Institute, Abbassia. Cairo.

Email

-

City

-

Orcid

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First Name

NADIA M

Last Name

IBRAHIM

MiddleName

-

Affiliation

Veterinary Serum and Vaccine Research Institute, Abbassia. Cairo.

Email

-

City

-

Orcid

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Volume

49

Article Issue

4

Related Issue

49663

Issue Date

2001-10-01

Receive Date

2024-08-06

Publish Date

2001-10-01

Page Start

563

Page End

573

Print ISSN

1110-1423

Online ISSN

2537-1045

Article File

VMJG_Volume 49_Issue 4_Pages 563-573.pdf

PDF . 3.8MB

Link

https://vmjg.journals.ekb.eg/article_372275.html

Detail API

https://vmjg.journals.ekb.eg/service?article_code=372275

Order

372,275

Type

Original Article

Type Code

544

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

PREPARATION AND LABORATORY EVALUATION OF LIVE ATTENUATED INFECTIOUS BURSAL DISEASE (IBD) VIRUS VACCINE FROM RECENT EGYPTIAN ISOLATE PROPAGATED ON CHICKEN EMBRYO FIBROBLAST (CEF)

Details

Type

Article

Created At

23 Dec 2024