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372272

PREPARATION AND EVALUATION OF AN INACTIVATED INFECTIOUS BURSAL DISEASE (IBD) VIRUS VACCINES FROM RECENT EGYPTIAN VIRUS ISOLATE ADJUVATED WITH NIGELLA SATIVA OIL.

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Last updated: 03 Jan 2025

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Virology and viral diseases

Abstract

Inactivated IBD virus vaccines were prepared from a recent Egyptian isolate and adjuvated with Nigella sativa oil. The first passages of propagat ed viruses in SPF- embryonated chicken eggs ( ECE), Vero and chicken embryo fibroblast (CEF ) cell cultures were inactivated with binary ethy- lenemine (BEI), and supplemented with Negilla sativa oil as adjuvant The prepared vaccines proved to be highly immu- nogenic and elicited high titers of neutralizing an- libodies (17-20 log 2 )at weekly interval till 7 months post-vaccination (PV) and high values of lymphocyte blastogenesis (0.598 versus control 06). Besides they were able to protect vaccinat- ed chickens (100 % protection) when challenged Days PV. The superior potential effect of these vaccines, when compared with imported one, may due to the use of recent local IBDV isolate and Nigella sativa oil for its non specific immune stimulation effect. In addition, the keeping quality of prepared vaccines proved to be sterile, safe, stable and potent when preserved at 4°C for 6 months (the end of the experiment) as they pro- duced 100 % and 80 % protection after 3 and 6 months of preservation respectively. Each chick received 0.3 ml as one dose subcutaneously. From these studies we conclude that the use of Nigella sativa oil as adjuvant is considered a new trend in preparing inactivated vaccines.

DOI

10.21608/vmjg.2001.372272

Authors

First Name

H

Last Name

MADBOULY

MiddleName

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Affiliation

Faculty of Veterinary Medicine, Cairo University, Beni-suef branch.

Email

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City

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Orcid

-

First Name

ENSAF M

Last Name

KHASHABAH

MiddleName

-

Affiliation

Serum and Vaccines Research Institute, Abbassia. Cairo.

Email

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City

-

Orcid

-

First Name

NADIA M

Last Name

IBRAHIM

MiddleName

-

Affiliation

Serum and Vaccines Research Institute, Abbassia. Cairo.

Email

-

City

-

Orcid

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Volume

49

Article Issue

4

Related Issue

49663

Issue Date

2001-10-01

Receive Date

2024-08-06

Publish Date

2001-10-01

Page Start

553

Page End

562

Print ISSN

1110-1423

Online ISSN

2537-1045

Article File

VMJG_Volume 49_Issue 4_Pages 553-562.pdf

PDF . 3.5MB

Link

https://vmjg.journals.ekb.eg/article_372272.html

Detail API

https://vmjg.journals.ekb.eg/service?article_code=372272

Order

372,272

Type

Original Article

Type Code

544

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

PREPARATION AND EVALUATION OF AN INACTIVATED INFECTIOUS BURSAL DISEASE (IBD) VIRUS VACCINES FROM RECENT EGYPTIAN VIRUS ISOLATE ADJUVATED WITH NIGELLA SATIVA OIL.

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Type

Article

Created At

23 Dec 2024