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371860

COMPARISON OF DNA PROBE TECHNIQUE AND C-ELISA FOR DETECTION OF ANAPLASMA MARGINALE IN CATTLE

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Last updated: 03 Jan 2025

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Bacteriology and bacterial diseases

Abstract

Through this study, recent and modern diagnostic Techniques have been used for diagnosis of cattle Anaplasmosis. Out of 255 cattle that were represented by 162 foreign and 93 native breeds, blood Samples were collected for separation of serum And DNA extraction using very simple and rapid Guanidine thiocyanate and celite method. This investigation, throws a spot light on, the application Of C-ELISA and nucleic acid hybridization using DNA labelled probe for identification of cattle Persistently infected with A. marginale. The naive MSP-5 is one of the outer membrane proteins (19 kDa) of A. marginale that was recognized by Mab and immue sera in C-ELISA. R-MSP-5 was Used as coating antigen and the format of C- ELISA was improved for detection of antibodies Against A. marginale in both acute and carrier anImals. The results of C-ELISA obtained through This work showed that 15.3% of the examined cattle were positive and also identified as 16.7% and 12.9% positive reactivity in foreign and native Breeds respectively. Non-radioactive msp-2 labeled probe was used in dot-blot hybridization to Diagnose bovine anaplasmosis. Only 20% of the Examined animals showed positive signals in dot Blot hybridization, double C-ELISA/dot-blot hybridization positivity was 34 cases proved that these animals were in acute and active stage of infection however, 17 animals showed positive reactivity only with dot blot hybridization. This Work is considered as the first trial for diagnosis of bovine anaplasmosis in Egypt using these recent and modern diagnostic techniques.

DOI

10.21608/vmjg.2001.371860

Authors

First Name

G

Last Name

EID

MiddleName

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Affiliation

Department of Microbiology and Immunology Faculty of Veterinary Medicine, Cairo University

Email

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City

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Orcid

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First Name

K

Last Name

ABD EL-HAMID

MiddleName

-

Affiliation

Department of Microbiology and Immunology Faculty of Veterinary Medicine, Cairo University

Email

-

City

-

Orcid

-

First Name

W

Last Name

GAD-EL SAID

MiddleName

-

Affiliation

Department of Microbiology and Immunology Faculty of Veterinary Medicine, Cairo University

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-

City

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Orcid

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Volume

49

Article Issue

1

Related Issue

49660

Issue Date

2001-01-01

Receive Date

2024-08-04

Publish Date

2001-01-01

Page Start

45

Page End

57

Print ISSN

1110-1423

Online ISSN

2537-1045

Article File

VMJG_Volume 49_Issue 1_Pages 45-57.pdf

PDF . 4.4MB

Link

https://vmjg.journals.ekb.eg/article_371860.html

Detail API

https://vmjg.journals.ekb.eg/service?article_code=371860

Order

371,860

Type

Original Article

Type Code

544

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

COMPARISON OF DNA PROBE TECHNIQUE AND C-ELISA FOR DETECTION OF ANAPLASMA MARGINALE IN CATTLE

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Type

Article

Created At

23 Dec 2024