Three experiments were conducted. In Experiment 1, ovaries (n=126) were collected in pairs From slaughtered anoestrus, early pregnant and cyclic buffaloes. Ovarian follicles (1-3, 4-9 and ( 210 mm diameter) were counted, aspirated and Oocytes were recovered and evaluated. In Experiment 2, ovaries were divided into 2 groups. Group 1, ovaries bearing a CL (n-74) and Group 2 non-bearing CL (n= 74), ovarian follicles (2-8 mm) were counted, aspirated and oocytes evaluated. Experiment 3, oocytes were recovered using aspiration or slicing methods. In all experiments, oocytes were classified into good, fair,poor and denuded. Results showed that the development of small and total ovarian follicles are continuous and independent in early pregnant or cyclic buffalo cows, however, it significantly decreased (P<0.01) in the ovaries from anoestrus buffaloes. Number of medium and large sized follicles significantly increased (P<0.01) in cyclic buffaloes on Days 10-16 and 17-22 of the oestrous cycle, while large follicles significantly decreased (P<0.01) in the ovaries of pregnant buffaloes. A significantly higher (P<0.01) percentage of poor and denuded oocytes were recovered from anoestrus and pregnant buffalo ovaries. While, the highest (P<0.01) percentage of good quality oocytes were recovered from ovaries of cyclic buffaloes on Days 1-3 and 10- 16 of the oestrous cycle, eliciting that the stage Of oestrous cycle is affecting the quality of buffalos oocytes. In addition, the presence of CL Stimulates the development of a significantly higher (P< 0.01) number ovarian follicles which produced a significantly (P<0.05) higher Number of good quality oocytes. Slicing of buffa-produced a significantly higher number of fair, poor and denuded oocytes. In conclusion, number of ovarian follicles and yield and quality of oocytes were affected by the reproductive status, stage of the oestrous cycle, presence of a CL or the methods of oocytes retrieval.