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376549

EVULATION OF INDIRECT ELISA TEST In DIAGNOSIS OF BRUCELLOSIS IN CAMELS

Article

Last updated: 03 Jan 2025

Subjects

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Tags

Bacteriology and bacterial diseases

Abstract

A serological survey was carried out on 365 camels (Camelus dromedaries, one humped camel) in Saudi Arabia. Out of these 250 were Imported from the Sudan, 80 from the Norther Abattoir and 35 blood samples were collected om local markets in Jeddah, Saudi Arabia. Examintion Of these samples with Standard tube wn (SAT), Rose Bengal plate test hay «nt acidified plate antigen test and rivanol test (Riv. T) was done.  and their Positive result were  (6.85%), (9.59%), (12.02%) and (7.67%) respectively. application of the indirect  ELISA on the same camel sera using antibovine, anti ovine amd antigoat conjugate revealed  positive results of (11.5 %), (7.4%) and (6.8) respectively. the technique of ELISA with anti bovine conjugate showed excellent agreement with BAPAT, as a sensitive test with Kappa values of 0.947 and good agreement with Riv .t. as a specific test, with K values of0.78. The ELISA with anti bovine conjugate was proved to be sensetive as well as specific test for examining camels for brucellosis. anti bovine conjugate in ELISA correlated very well with camel sera than the antiovine or antigoat conjugate. two brucella isoletes were obtained from five aborted foeti. while no brucella isolates were obtained from 35 camel milk samples. the isolated strains proved to be brucella melitensis biovar3.

DOI

10.21608/vmjg.2000.376549

Authors

First Name

M

Last Name

HAMDY

MiddleName

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Affiliation

Department of Brucellosis, Animal Health Research Institute Dokki, Giza, Egypt

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City

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Orcid

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Volume

48

Article Issue

4

Related Issue

49644

Issue Date

2000-10-01

Receive Date

2024-08-27

Publish Date

2000-10-01

Page Start

467

Page End

477

Print ISSN

1110-1423

Online ISSN

2537-1045

Link

https://vmjg.journals.ekb.eg/article_376549.html

Detail API

https://vmjg.journals.ekb.eg/service?article_code=376549

Order

376,549

Type

Original Article

Type Code

544

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

EVULATION OF INDIRECT ELISA TEST In DIAGNOSIS OF BRUCELLOSIS IN CAMELS

Details

Type

Article

Created At

23 Dec 2024