Apramycin (20 mg/kg b. wt.) was administered by intravenous, oral and intramuscular routes in healthy chickens. Following intravenous injection, the blood concentration curve was exponential. The kinetic parameters showed an elimination half-life of 5.97 +0.07 hours' apparent volume of distribution 13.3320.25 L. / kg. and clearance rate of 1.55+0.04 ml/kg/min. Following oral and intramuscular administration of apramycin reached its maximum concentration 4.4620.07 and 5.67+0.14 ug/ml at maximum time. 41.7722.89 and 42.81+5.89 minutes, with interval between doses 5.12+0.04 and 13.55+0.40 respectively. Apramycin was distributed in all tissues with withdrawal time of 3 days following oral and intramuscular administration for 5 days.